One-step PCR: A novel protocol for determination of pfhrp2 deletion status in Plasmodium falciparum
نویسندگان
چکیده
منابع مشابه
Deletion mutagenesis of large (12-kb) plasmids by a one-step PCR protocol.
Engendering deletions in genes and then assaying the functional consequences is a useful approach for mapping cis-acting regulatory elements. There are several different strategies for generating large deletions. One of the most convenient ways is to use two restriction enzymes to remove an internal fragment. However, in many cases, convenient enzyme sites are not available. Another approach is...
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BACKGROUND Plasmodium falciparum encoded histidine rich protein (HRP2) based malaria rapid diagnostic tests (RDTs) are used in India. Deletion of pfhrp2 and pfhrp3 genes contributes to false negative test results, and large numbers of such deletions have been reported from South America, highlighting the importance of surveillance to detect such deletions. METHODS This is the first prospectiv...
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A number of studies have analyzed the performance of malaria rapid diagnostic tests (RDTs) in Colombia with discrepancies in performance being attributed to a combination of factors such as parasite levels, interpretation of RDT results and/or the handling and storage of RDT kits. However, some of the inconsistencies observed with results from Plasmodium falciparum histidine-rich protein 2 (PfH...
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Most Plasmodium falciparum-detecting rapid diagnostic tests (RDTs) target histidine-rich protein 2 (PfHRP2). However, P. falciparum isolates with deletion of the pfhrp2 gene and its homolog gene, pfhrp3, have been detected. We carried out an extensive investigation on 365 P. falciparum dried blood samples collected from seven P. falciparum endemic sites in Colombia between 2003 and 2012 to gene...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0236369